Publications

UK NEQAS for Leucocyte Immunophenotyping has contributed to the scientific literature for over three decades, advancing standards in flow cytometry, molecular haemato-oncology, and external quality assessment/proficiency testing worldwide.

Our publications span consensus guidelines, interlaboratory studies, and methodological research – covering areas from CD4+ T cell counting and CD34+ stem cell enumeration to PNH testing, measurable residual disease in haematological malignancies, and chimerism monitoring after HSCT. These collaborative works reflect our commitment to improving laboratory accuracy and patient outcomes through rigorous external quality assessment.

United Kingdom National External Quality Assessment Service for Leucocyte Immunophenotyping Chimerism Working Group. Monitoring of chimerism following allogeneic haematopoietic stem cell transplantation (HSCT): technical recommendations for the use of short tandem repeat (STR) based techniques, on behalf of the United Kingdom National External Quality Assessment Service for Leucocyte Immunophenotyping Chimerism Working Group

Clark JR, Scott SD, Jack AL, Lee H, Mason J, Carter GI, Pearce L, Jackson T, Clouston H, Sproul A, Keen L, Molloy K, Folarin N, Whitby L, Snowden JA, Reilly JT, Barnett D

Br J Haematol. 2015 Jan;168(1):26-37. doi: https://doi.org/10.1111/bjh.13073. Epub 2014 Aug 22. PMID: 25145701.

Standardizing leucocyte PNH clone detection: an international study

Fletcher M, Sutherland DR, Whitby L, Whitby A, Richards SJ, Acton E, Keeney M, Borowitz M, Illingworth A, Reilly JT, Barnett D

Cytometry B Clin Cytom. 2014 Sep;86(5):311-8. doi: https://doi.org/10.1002/cyto.b.21174. Epub 2014 Apr 9. PMID: 24715466.

Systematic review of the use of dried blood spots for monitoring HIV viral load and for early infant diagnosis

Smit PW, Sollis KA, Fiscus S, Ford N, Vitoria M, Essajee S, Barnett D, Cheng B, Crowe SM, Denny T, Landay A, Stevens W, Habiyambere V, Perriens JH, Peeling RW

PLoS One. 2014 Mar 6;9(3):e86461. doi: https://doi.org/10.1371/journal.pone.0086461. PMID: 24603442; PMCID: PMC3945725.

Systematic review of the performance of HIV viral load technologies on plasma samples

Sollis KA, Smit PW, Fiscus S, Ford N, Vitoria M, Essajee S, Barnett D, Cheng B, Crowe SM, Denny T, Landay A, Stevens W, Habiyambere V, Perrins J, Peeling RW

PLoS One. 2014 Feb 18;9(2):e85869. doi: https://doi.org/10.1371/journal.pone.0085869. PMID: 24558359; PMCID: PMC3928047.

ICSH/ICCS Working Group. Validation of cell-based fluorescence assays: practice guidelines from the ICSH and ICCS – part IV – postanalytic considerations

Barnett D, Louzao R, Gambell P, De J, Oldaker T, Hanson CA

Cytometry B Clin Cytom. 2013 Sep-Oct;84(5):309-14. doi: https://doi.org/10.1002/cyto.b.21107. PMID: 24022853.

Comparison of methodological data measurement limits in CD4⁺ T lymphocyte flow cytometric enumeration and their clinical impact on HIV management

Whitby L, Whitby A, Fletcher M, Helbert M, Reilly JT, Barnett D

Cytometry B Clin Cytom. 2013 Jul-Aug;84(4):248-54. doi: https://doi.org/10.1002/cyto.b.21094. Epub 2013 Apr 23. PMID: 23788473.

VERITAS?: A time for VERIQAS™ and a new approach to training, education, and the quality assessment of CD4+ T lymphocyte counting (I)

Barnett D, Whitby L, Wong J, Louzao R, Reilly JT, Denny TN

Cytometry B Clin Cytom. 2012 Mar;82(2):93-100. doi: https://doi.org/10.1002/cyto.b.20624. Epub 2011 Oct 13. PMID: 21998025; PMCID: PMC4126193

ISHAGE protocol: are we doing it correctly?

Whitby A, Whitby L, Fletcher M, Reilly JT, Sutherland DR, Keeney M, Barnett D

Cytometry B Clin Cytom. 2012 Jan;82(1):9-17. doi: https://doi.org/10.1002/cyto.b.20612. Epub 2011 Sep 13. PMID: 21915992.

CD4 immunophenotyping in HIV infection

Barnett D, Walker B, Landay A, Denny TN

Nat Rev Microbiol. 2008 Nov;6(11 Suppl):S7-15. doi: https://doi.org/10.1038/nrmicro1998. PMID: 18923413; PMCID: PMC2728050.

Long-term stabilized blood samples as controls for flow cytometric HLA-B27 screening: a feasibility study

Levering WH, Wind H, Granger V, Sintnicolaas K, Hooijkaas H, Reilly JT, Gratama JW, Barnett D

Cytometry B Clin Cytom. 2008 May;74(3):169-81. doi: https://doi.org/10.1002/cyto.b.20388. PMID: 18200592